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Pentoxifylline‐mediated reduction of superoxide anion production by human spermatozoa
Author(s) -
Gavella M.,
Lipovac V.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1992.tb02606.x
Subject(s) - superoxide , pentoxifylline , chemistry , superoxide dismutase , sperm , andrology , human fertilization , in vitro , biochemistry , medicine , enzyme , anatomy
Summary. The release of superoxide anions from sperm suspensions prepared for in vitro fertilization was measured by superoxide dismutaseinhibitable reduction of ferricytochrome C in response to phorbol myristate acetate. The superoxide anion production was found to be within the range of 0.21‐3.76 nmoles O ‐ 2 per 10 6 spermatozoa. Spermatozoa pre‐incubated in the presence of 3.7 or 10 mM of pentoxifylline for 30 min at 37 °C released less ( P <0.001) superoxide anions than did the untreated‐control spermatozoa. It has been concluded that the inhibitory effect of pentoxifylline on superoxide anion production may be a factor contributing to the improvement of sperm preparation before insemination, and may, thereby, increase the fertility potential.