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Germ cell DNA quantification shortly after IR laser radiation
Author(s) -
Bermúdez D.,
Carrasco F.,
Diaz F.,
PerezdeVargas I.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1991.tb02566.x
Subject(s) - dna , germ cell , spermatid , andrology , laser , dna damage , biology , chemistry , irradiation , radiation , germ , cell cycle , cell , microbiology and biotechnology , spermatogenesis , biochemistry , medicine , optics , gene , physics , nuclear physics
Summary. The immediate effect of IR laser radiation on rat germ cells was studied by cytophotometric quantification of the nuclear DNA content in testicular sections. Two different levels of radiation were studied: one according to clinical application (28.05 J/cm 2 ) and another known to increase the germ cell number (46.80 J/cm 2 ). The laser beam induced changes in the germ cell DNA content depending on the cell type, the cell cycle phase and the doses of radiation energy applied. Following irradiation at both doses the percentage of spermatogonia showing a 4c DNA content was increased, while the percentage of these with a 2c DNA content was decreased. Likewise, the percentages of primary spermatocytes with a DNA content equal to 4c (at 28.05 J/cm 2 ), between 2c and 4c (at 46.80 J/cm 2 ) and higher than 4c (at both doses) were increased. No change in the mean spermatid DNA content was observed. Nevertheless, at 46.80 J/cm 2 the percentages of elongated spermatids with a c or 2c DNA content differed from the controls. Data show that, even at laser radiation doses used in therapy, the germ cell DNA content is increased shortly after IR laser radiation.