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Radio‐immuno binding test for anti‐sperm antibody detection: analysis and critical revision of various methodological steps
Author(s) -
Gandini L.,
Lombardo F.,
Lenzi A.,
Dondero F.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1991.tb02501.x
Subject(s) - sperm , radioimmunoassay , antibody , computational biology , immunology , biology , medicine , andrology
Summary. The authors report the results of a methodological study to optimize a radioimmunoassay to detect anti‐sperm antibodies. They describe here the definitive methodology with all the data relating to the laboratory trials conducted at each step of the technique. Results seem to demonstrate that this radioimmunological method is sufficiently simple, specific, sensitive and reliable for use in a highly specialized laboratory of reproductive immunology. Furthermore, this type of analytical approach, whilst indicating once more the difficulty of the technical study of anti‐sperm autoimmunity, confirms (with concrete data) the necessity of being extremely “prudent” in evaluating the results of this kind of anti‐sperm antibody test.