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Concentrating human sperm before cryopreservation
Author(s) -
Kobayashi T.,
Kaneko S.,
Hara I.,
Park Y. J.,
Sato H.,
Ohno T.,
Nozawa S.
Publication year - 2009
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/j.1439-0272.1991.tb02487.x
Subject(s) - semen , motility , sperm , andrology , sperm motility , cryopreservation , sperm quality , sperm washing , biology , semen cryopreservation , sperm bank , centrifugation , semen quality , chemistry , medicine , biochemistry , insemination , microbiology and biotechnology , embryo
Summary. To improve the quality of cryo‐preserved sperm, ejaculated semen was concentrated prior to freezing by means of continuous‐step density gradient centrifugation. Freezing was simplified by employing liquid nitrogen vapor with KS‐II cryo‐medium. The original semen ( n = 32, 41 ± 13 × 10 6 /ml, 17 ± 11% motility) was improved by the processing to be 68 ± 29 × 10 6 /ml, 55 ± 19% motility. Even after the specimens were thawed, sperm concentrations were similar to those of the original semen and 39 ± 18% motility remained with a mean survival rate of 74 ± 7.7%. The improved sperm motility and higher survival rate resulted in better post‐thaw sperm quality than those of the original semen.