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Expression of M n‐ SOD , i NOS and e NOS m RNA s in Osteoblasts from the Maxilla of Osteopetrotic Mice
Author(s) -
Kuwana M.,
Watanabe H.,
Abe S.,
Yanagisawa T.,
Sasaki J.
Publication year - 2013
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.2012.01173.x
Subject(s) - chemistry , enzyme , osteoblast , microbiology and biotechnology , biochemistry , medicine , biology , in vitro
Summary Active oxygens and free radicals are involved in the metabolism and clinical conditions of tissues; however, little is known about the localisation and expression levels of associated enzymes. The expressions of active oxygens, free radicals and associated enzymes are reported to be site‐specific; therefore, the expression states of free radical enzymes differ between sites, even within the same cell. In particular, there has been no report concerning the catabolic enzymes of active oxygens in osteoblasts of the maxilla, other than normal osteoblasts that were weakly positive by immunohistochemical staining. We conducted this study to elucidate the relationship between osteodystrophy and radical‐associated enzymes by investigating m RNA s of enzymes associated with active oxygens and free radicals using osteoblasts from the maxilla of normal and osteopetrotic model (op/op) mice. In op/op mouse maxilla osteoblasts, m RNA s of M n‐ SOD , i‐ NOS and e‐ NOS were strongly positive. M n‐ SOD and i NOS enzymes were considered to be highly expressed in osteoblasts of the narrowed medullary cavity of this bone.