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Confocal Imaging of Trans‐epithelial Trafficking by Immune and Umbilical Cord Stem Cells in the Neonatal Porcine Intestine
Author(s) -
Miller D.,
Packthongsuk K.,
Rathbun T.,
Boyle D.,
Troyer D.,
Davis D. L.
Publication year - 2012
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.2012.01157.x
Subject(s) - umbilical cord , cord lining , stem cell , immune system , microbiology and biotechnology , confocal , confocal microscopy , pathology , biology , immunology , adult stem cell , medicine , embryonic stem cell , biochemistry , geometry , mathematics , gene
Summary Maternal colostral leucocytes ( CL ) and peripheral blood mononuclear cells ( PBMC ) enter the neonatal circulation after ingestion in pigs and cattle. Porcine umbilical cord matrix stem cells ( PUC s) are relatively non‐immunogenic after initial allogeneic transplantation. Using intestinal explant cultures incubated with labelled cells and confocal microscopy, we demonstrated trans‐epithelial trafficking of exogenous CL , PBMC and PUC s below the luminal surface after 72 h of culture. We orally administered PBMC and PUC s to pre‐colostral neonatal pigs and tracked their location 8 or 24 h later. Both PBMC and PUC s were found in the intestinal wall of all samples. Exposure to 25% of acellular colostrum had no detected effect on trafficking. Labelled PUC s and PBMC were detected on the surface of the epithelium and in the lamina propria 8 h post‐treatment and PBMC were also in the superficial submucosa. At 24 h, PUC s and PBMC were observed on the surface of the epithelium, in the lamina propria, superficial submucosa and deep submucosa. Our findings show the potential of PUC s for allogeneic engraftment in the neonatal intestine and may lead to cell‐based delivery of therapeutics.