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Retrograde Tracing with Fluorescent Microspheres Reveals Bifurcating Projections from Central Retina to Tectum and Thalamus in Chicks
Author(s) -
Cao J.,
Naito J.,
Chen Y.
Publication year - 2012
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.2011.01131.x
Subject(s) - tectum , thalamus , retina , retrograde tracing , anatomy , biology , neuroscience , retinal ganglion cell , midbrain , central nervous system
Summary The goal of this study is to demonstrate the dual‐projection pattern of retinal ganglion cells ( RGC s) projecting to the tectum and visual thalamus in chick using retrograde fluorescent tracers and also to define the morphological properties of these RGC s with dual projections by intracellular injection of L ucifer Y ellow ( LY ) combined with immunohistochemistry. Thirty‐two chicks received double injections of green and red fluorescent microspheres into their thalamus and tectum in the same side. In the central retina, most of the labelled RGC s were tec‐ RGC s ( RGC s projecting to the tectum), a quarter was tha‐ RGC s ( RGC s projecting to the thalamus), and almost all of the tha‐ RGC s were double‐labelled RGC s. An intracellular injection of LY into the double‐labelled RGC s showed all six groups of RGC s without specific populations in each group (J. Comp. Neurol., 2004, 469: 360). These dendritic patterns were mostly mono‐ and bistrata, which extended horizontally in the deeper part of the inner plexiform layer.