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Neurochemical Properties of Aquaporin 1‐Expressing Sensory Neurons from the Ovine Trigeminal Ganglion
Author(s) -
Arciszewski M. B.
Publication year - 2012
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.2011.01122.x
Subject(s) - trigeminal ganglion , calcitonin gene related peptide , galanin , ganglion , anatomy , dorsal root ganglion , sensory system , substance p , population , nodose ganglion , sensory neuron , biology , neuropeptide , neuroscience , chemistry , medicine , receptor , vagus nerve , environmental health , stimulation
Summary Aims of the present study were to investigate the distribution and morphology of aquaporin 1‐immunoreactive ( AQP 1‐ IR ) neurons in the sensory ganglia of the sheep. Double immunohistochemical staining was applied to figure out whether substance P ( SP ), calcitonin gene‐related peptide ( CGRP ) and galanin are present in AQP 1‐bearing primary afferent neurons. The expression of AQP 1 was present only in trigeminal ganglion, whereas in nodose ganglion, jugular ganglion as well as C 1 – C 7 dorsal root ganglia no presence of AQP 1 was found. In trigeminal ganglion, 15.4 ± 2.3% of Hu C / D ‐ IR neurons (pan‐neuronal marker) showed the presence of AQP 1. The vast majority of AQP 1‐ IR trigeminal sensory neurons (approximately 69.6 ± 3.3%, n = 5) were classified as middle in size, 28.6 ± 3.0% of AQP 1‐ IR neurons were small and only 1.8 ± 0.6% of AQP 1‐positive neurons were large in size. Amongst the population of AQP 1‐ IR trigeminal neurons as many as 58.5 ± 3.9% were immunopositive to SP , 30.7 ± 2.3% showed the presence of CGRP and 10.9 ± 0.2% coexpressed galanin. In trigeminal ganglion, SP ‐ IR as well as CGRP ‐ IR (but not galanin‐ IR ) nerve fibres were found in close neighbourhood of AQP 1‐ IR neurons. It is concluded that AQP 1 is present in certain neuronal subsets of the ovine trigeminal ganglion; however, the exact role of this water channel has to be elucidated.