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The Cocaine‐ and Amphetamine‐regulated Transcript (CART) Immunoreactivity in the Amygdala of the Pig
Author(s) -
Równiak M.,
Robak A.,
BogusNowakowska K.,
Wasilewska B.,
Najdzion J.,
Majewski M.
Publication year - 2010
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.2010.01006.x
Subject(s) - cocaine and amphetamine regulated transcript , cart , vasoactive intestinal peptide , neuropeptide , amygdala , neurotensin , biology , endocrinology , cholecystokinin , population , basolateral amygdala , neuropeptide y receptor , medicine , substance p , golgi apparatus , nucleus , hypothalamus , chemistry , microbiology and biotechnology , receptor , biochemistry , endoplasmic reticulum , mechanical engineering , environmental health , engineering
With 5 figures and 1 table Summary The distribution and morphology of neurons containing cocaine‐ and amphetamine‐regulated transcript (CART) was investigated in the pig amygdala. CART‐ immunoreactive (CART‐IR) cell bodies were rarely observed in the pig amygdala and most often they were present in the posterior (small‐celled) parts of the basolateral and basomedial nuclei. In all other subdivisions only a small number of randomly scattered pericarya were present. In every region studied the CART‐IR neurons formed a heterogeneous population consisting mostly of small, rounded or slightly elongated cell bodies, with a few poorly branched, smooth dendrites. In general, the morphological features of these cells clearly resembled non‐pyramidal Golgi type II interneurons. Some randomly scattered CART‐IR cell bodies were significantly larger and they demonstrated features of pyramidal‐like Golgi type I projecting neurons. The highest densities of CART‐IR fibres were evident within the central and medial nuclei. Moderate to high expression was found within the large‐celled part of the basolateral nucleus and moderate to low levels in the lateral, basomedial and cortical nuclei. The routine double‐labelling studies with antisera directed against CART and somatostatin (SOM), or neuropeptide Y (NPY), or cholecystokinin (CCK), or vasoactive intestinal peptide (VIP), or substance P (SP) demonstrated that, in general, these peptides do not co‐exist in the CART‐IR neurons. However, small subpopulations of the CART‐IR fibres contained SOM, CCK, VIP or SP together.