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MHC Class II+ (HLA‐DP‐like) Cells in the Cow Reproductive Tract: II. Immunolocalization of MHC Class II+ Cells in Oviduct and Vagina
Author(s) -
Eren U.,
Kum S.,
Sandıkçı M.,
Eren V.,
Ilhan F.
Publication year - 2009
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.2009.00940.x
Subject(s) - oviduct , ampulla , lamina propria , muscularis mucosae , vagina , biology , mhc class ii , pathology , population , epithelium , immune system , andrology , major histocompatibility complex , anatomy , endocrinology , immunology , medicine , genetics , environmental health
Summary The aim of this study was to determine and examine the distribution of major frequency MHC II+ cells in the oviduct and vagina of cows during the oestrous and dioestrus phases. Right oviduct (ampulla, isthmus) and vaginal samples taken from a total of twenty seven multiparous cows were used. Tissue samples were processed to obtain both cryostat and paraffin sections. Sections were stained immunocytochemically using StreptABC method using a specific monoclonal antibody to MHC II+ cell population. Intra‐epithelial and subepithelial areas along with lamina propria, muscularis mucosae and serosa of both ampulla and isthmus and intra‐epithelial/subepithelial areas and mucosae of vagina were examined for the presence of MHC II+ cells. The density of immune positive cells was determined using a subjective scoring system. MHC II+ cells were demonstrated in all areas examined in both oestrus and dioestrus. In oestrus, the density of MHC II+ cells decreased in subepithelial areas (in between the epithelial cells and the basal membrane) of isthmus, whereas the density of immune positive cells was increased in muscularis mucosae of isthmus ( P < 0.05), lamina propria and muscularis mucosae of ampulla ( P < 0.05) as well as in the mucosae of vagina ( P ≤ 0.005). This study indicates that the density of MHC II+ cells observed in the oviduct and vagina increases in the majority of areas examined due to the effect of oestrogen.