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Histological Evaluation of the Sheep’s Cervical Spine Segment 3/4 – A Histomorphological and Histomorphometrical Study
Author(s) -
Koch C.,
Kandziora F.,
Huenigen H.,
Plendl J.
Publication year - 2008
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.2007.00829.x
Subject(s) - trichrome , anatomy , cartilage , pathology , intervertebral disc , safranin , biology , trichrome stain , medicine , immunohistochemistry , h&e stain
Summary The 2‐year‐old sheep is a frequently used model in spinal orthopaedic research; however, so far no investigations have been performed on physiological tissue of untreated animals. In this study, the cervical spine segment 3/4 of eight 2‐year‐old female Merino sheep was evaluated histologically and histomorphometrically. Specimens were stained with Masson‐Goldner‐Trichrome, Safranin‐Orange/Lightgreen, Safranin‐Orange/von Kossa and Astrablue. Analysis of bone mass, mass of cartilage and mineralized cartilage in a defined region of interest was performed. A new finding of the study was that – although 2‐year‐old sheep is regarded as adult – tissue of the growth plate was still detected. Moreover, this study revealed that in the nucleus pulposus of the 2‐year‐old sheep notochordal cells are still existent, suggesting a complex remodelling of the nucleus pulposus in sheep.