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Perineal Nets of Proteoglycans and Glycoproteins in Albino Rat Hippocampus
Author(s) -
Mubarak W.,
Saleh M. N.,
Sayed R.,
Ohtsuka A.,
Taguchi T.,
Murakami T.
Publication year - 2005
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.2005.00669_78.x
Subject(s) - perineuronal net , hippocampal formation , hippocampus , neuroscience , glycoconjugate , gabaergic , staining , chemistry , biology , parvalbumin , inhibitory postsynaptic potential , biochemistry , genetics
Perineuronal nets (PNs) of condensed extracellular matrix (ECM) have been shown to characterize the microenvironment of individual neurons and the chemoarchitecture of some brain regions. In the present study, PNs in the hippocampus were visualized with a cationic iron colloid method for sulphated proteoglycan content and a plant lectin from Vicia villosa agglutinin (VVA) for N‐acetylgalactosamine containing glycoconjugates. The ECM molecules were organized in reticular coats (PNs) around non‐pyramidal cells in the Ammons horn (Corneu Ammons, CA) and subicular region, in addition to pyramidal neurons located in CA2 and CA3 regions. CA2 stratum pyramidale exhibited the most intense staining of its PNs and a diffuse intervening neutrophil labelling, while CA3 region showed a graded fashion of staining intensity. The subiculum displayed intensely stained perineuronal coats. Notably, the hippocampal perineuronal nets revealed overlapped staining characteristics with both staining methods. However, cell coats of the subicular neurons showed various degrees of labelling characteristics with both markers. It is suggested that the PNs in the hippocampus are correlated with the fast spiking inhibitory GABAergic neurons and their target pyramidal cells, and this is important to keep the excitatory elements under control and therefore control the information processes within the hippocampus.