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Sirius Red is able to Selectively Stain Eosinophil Granulocytes in Bovine, Ovine and Equine Cervical Tissue
Author(s) -
Wehrend A.,
Hetzel U.,
Huchzermeyer S.,
Klein C.,
Bostedt H.
Publication year - 2004
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.2004.00532.x
Subject(s) - sirius red , staining , stain , eosinophil , sirius , fixation (population genetics) , pathology , infiltration (hvac) , neutral red , biology , eosinophilic , andrology , chemistry , anatomy , medicine , immunology , biochemistry , in vitro , stars , physics , astronomy , cytotoxicity , asthma , gene , thermodynamics
Summary The aim of the present study was to examine the suitability of sirius red staining for selective light microscopic demonstration of eosinophil granulocytes in cervical tissue of mares, cows and sheeps. For this purpose, tissue was fixed in 4% neutral buffered formol or in Bouin's solution. Paraffin sections of 5‐ μ m thickness were stained with sirius red. In cows, mares and sheeps a selective distinction of eosinophilic infiltration is successful after both fixation methods.