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An Immunohistochemical Study of Gastrointestinal Endocrine Cells in the BALB/c Mouse
Author(s) -
Ku S. K.,
Lee H. S.,
Lee J. H.
Publication year - 2004
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.2004.00508.x
Subject(s) - immunohistochemistry , enteroendocrine cell , endocrine system , balb/c , biology , pathology , medicine , immunology , hormone , immune system
Summary The distributions and frequencies of some endocrine cells in the eight portions of the gastrointestinal tract (GIT) of BALB/c mouse were studied. Endocrine cells were stained using immunohistochemical method with seven types of antisera against bovine chromogranin (BCG), serotonin, gastrin, cholecystokinin (CCK)‐8, somatostatin, glucagon and human pancreatic polypeptide (HPP), and the regional distributions and their relative frequencies were observed in the eight portions of the GIT of BALB/c mice. All seven types of immunoreactive (IR) cells were identified. Most of the IR cells in the intestinal portion were generally spherical or spindle in shape (open type cell) while round‐shaped cells (closed type cell) were found in the intestinal gland and stomach regions occasionally. Their relative frequencies varied according to each portion of the GIT. BCG‐IR cells were observed throughout the whole GIT except for the rectum and they were most predominant in the pylorus. Serotonin‐IR cells were detected throughout the whole GIT and they showed the highest frequency in the fundus. Gastrin‐ and CCK‐IR cells were restricted to the pylorus and duodenum with a majority in the pylorus and rare or a few frequencies in the duodenum. Compared with other mammals, somatostatin‐IR cells were restricted to the fundus and pylorus with a few frequencies, respectively. In addition, glucagon‐ and HPP‐IR cells were restricted to the fundus and duodenum, respectively, with relative low frequencies. Some species‐dependent unique distributions and frequencies of endocrine cells were observed in the GIT of BALB/c mouse compared with other rodents.