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Distribution of Lymphocyte Subsets in the Small Intestine Lymphoid Tissue of 1‐Month‐Old Lambs
Author(s) -
Corpa J M,
Juste R A,
Marín J. F. García,
Reyes E,
González J,
Pérez V
Publication year - 2001
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.2001.00312.x
Subject(s) - lamina propria , biology , lymphatic system , pathology , peyer's patch , lymphocyte , lymph node , cd8 , immunohistochemistry , antibody , b cell , mesenteric lymph nodes , t lymphocyte , monoclonal antibody , cd3 , immune system , immunology , epithelium , medicine
Distribution of lymphocyte subpopulations along the small intestine lymphoid tissue has been examined in 1‐month‐old lambs using flow cytometric and immunohistochemical techniques. Monoclonal antibodies against CD4, CD8, γδ, CD45R and B receptors have been employed in samples from continuous ileal Peyer’s patch (IPP), discrete jejunal Peyer’s patches (JPP), ileocaecal valve lymphoid tissue (ICVPP), mesenteric lymph node (MLN) and intra‐epithelial (IEL) and lamina propria (LPL) lymphocytes. Histological studies were also done. Differences in the lymphocyte distribution have been observed between some of the regions examined, especially between IPP and JPP for most of the markers. A remarkable feature was the existence of morphological and lymphocyte distribution differences between ICVPP and IPP, locations that had been traditionally considered as similar. The antibody against CD45R receptor used in this study, that was supposed to mark B cells and some T cells, detected cell populations located in the dome of the follicles in all the samples, whereas the centre was negative. Lymphocytes positive to the B marker employed were located mainly in the centre, suggesting that both antibodies would mark B cells in different maturation status.