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Detection of the Ki‐67 Proliferation Associated Nuclear Epitope in Normal Canine Tissues Using the Monoclonal Antibody MIB‐1
Author(s) -
Laprie C.,
Abadie J.,
Amardeilh M. F.,
Raymond I.,
Delverdier M.
Publication year - 1998
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.1998.tb00189.x
Subject(s) - epitope , monoclonal antibody , immunohistochemistry , ki 67 , proliferation marker , proliferating cell nuclear antigen , pathology , microbiology and biotechnology , biology , antigen , antibody , monoclonal , immunology , medicine
Summary This report describes the immunohistochemical detection of the Ki‐67 proliferation associated nuclear epitope by means of the MIB‐1 monoclonal antibody (mAb) in paraffin tissue sections from nine samples of 16 tissues obtained from nine dogs. Three parameters were considered: the localization of the cells expressing the Ki‐67 epitope, the cytological characteristics of the Ki‐67 expression, and the proliferative activity of some of the tissues measured by means of the proliferative index (percentage of Ki‐67 positive cells measured on 500 and 1000 cells). The MIB‐1 mAb reacts with the nuclei of proliferating cells, as in humans. The proliferative index was far from representative, but we were able to give a range of values concerning the proliferative activity of normal tissues. This study serves as a basis for the expression of the Ki‐67 antigen by normal canine tissues in order to visualize the proliferative compartments and, thus, allows its application as a proliferative marker in routine veterinarian histopathology.