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Actinfilaments in the Ruminal Epithelium of Cattle
Author(s) -
Kressin M.,
Koob B.,
Schnorr B.
Publication year - 1993
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.1993.tb00352.x
Subject(s) - immunogold labelling , tannic acid , phalloidin , actin , cytoskeleton , microbiology and biotechnology , epithelium , biology , glutaraldehyde , biophysics , chemistry , anatomy , cell , biochemistry , ultrastructure , botany , genetics , chromatography
Summary Phalloidin and immunogold labeling were used to localize actinfilaments in the ruminal pilar epithelium of adult catde on the light and electron microscopic level. Incubation with rhodaminyl‐phalloidin permitted an overview of general actin distribution pattern. Distinct subcellular localization of actin was revealed with the biotin‐streptavidin bridge technique in a postembedding procedure. Superior fixation of membranes and filaments was achieved with tannic acid‐glutaraldehyde. Surface‐etching prior to immunoincubation ensured restoration of actin immunoreactivity. Patterns of distribution in non‐keratinized and keratinized epithelial cells as well as in pericytes and endothelial cells point to cell‐specific cytoskeletal and motile functions of actinfilaments.