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Original Papers
Author(s) -
Lehmann Margot,
Russe Imogen,
Sinowatz Fred
Publication year - 1992
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.1992.tb00459.x
Subject(s) - lectin , trophoblast , fucose , incubation , embryo , biology , microbiology and biotechnology , staining , cell , placentation , galactose , chemistry , biochemistry , glycoprotein , placenta , fetus , genetics , pregnancy
Nachweis von Lektinbindungsstellen am Trophoblast des Rindes während der Frühgravidität* Summary Histotopographical distribution of lectin binding sites in trophoblasts of bovine embryos In the present study we report on the histotopographical distribution of lectin binding sites in the trophoblasts of day 18 to day 40 bovine embryos, using the FITC‐labeled lectins BPA, Con A, DBA, GS I, GS II, MPA, PNA, SBA, UEA I and WGA. Lectin binding sites localized in giant binucleate cells differ from those localized in uninucleate cells, indicating changes in the biochemical structure of cell surfaces taking place during differentiation. In the trophoblast of the day 40 embryo, a distinct staining of uninucleate cells was seen after incubation with GS I, Con A and MPA, demonstrating N‐acetylgalactosamin (GS I), Mannose (Con A) and Galactose (MPA) moieties, whereas giant binucleate cells showed intense reactions after incubation with DBA and WGA, indicating presence of N‐acetylgalactosamin (DBA) and N‐acetylglucosamin (WGA). GS II (specific for N‐acetylglucosamin), SBA (specific for N‐acetylgalactosamin) and UEA I (specific for L‐Fucose) showed no affinity toward any of the examined tissues. We assume, that carbohydrate moieties in trophoblast cells play an important role in fetomaternal cell‐cell adhesion and cell migration during implantation and placentation period.