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Cytological, Immunocytochemical and Ultrastructural Study of the Adenohypophyseal Pars Distalis of the Kid ( Capra hircus ): The TSH cell
Author(s) -
Gomez M. A.,
Navarro J. A.,
Gomez S.,
Camara P.,
Gomez J. C.,
Bernabé A.
Publication year - 1989
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/j.1439-0264.1989.tb00604.x
Subject(s) - capra hircus , ultrastructure , pathology , immunocytochemistry , biology , medicine , zoology
Summary The structure and ultrastructure of the adenohypophyseal pars distalis in kids of the Murciano‐granadiana breed (18 males and 12 females), aged 2–3 months and with an average carcass weight of 8 kg has been studied. Techniques of staining (Tetrachrome Herlant's, and Cleveland‐Wolfe) and histochemistry (PAS, PAS‐OG and BA‐PAS‐OG) contrasted with immunolabelling (PAP) have been used. In addition an ultrastructural study has been made and nucleus and cytoplasm, secretory granules, mitochondria and lysosomes have been measured with a semiautomatic image analyzer (IBAS‐1). TSH cells are found in sagittal section in the anterior area and in an antero‐caudodorsal band, and transversally in the ventral and medial region; they are 6% and their average size is 14.15 um. Ultrastructurally they are a single cellular type with spherical granules whose size is 195nm in males and 149 in females; these granules are characterized by a clear halo and a variable electronic density. The rough endoplasmic reticulum is found as slightly enlarged vesicles with a homogeneous and moderately electrodense content or in concentric stratifications.