Premium
Functional analysis of endo‐1,4‐β‐glucanases in response to Botrytis cinerea and Pseudomonas syringae reveals their involvement in plant–pathogen interactions
Author(s) -
Finiti I.,
Leyva M. O.,
LópezCruz J.,
Calderan Rodrigues B.,
Vicedo B.,
Angulo C.,
Bennett A. B.,
Grant M.,
GarcíaAgustín P.,
GonzálezBosch C.
Publication year - 2013
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/j.1438-8677.2012.00701.x
Subject(s) - callose , biology , jasmonic acid , arabidopsis thaliana , pseudomonas syringae , arabidopsis , biotic stress , abiotic stress , microbiology and biotechnology , mutant , brassinosteroid , plant hormone , auxin , botany , cell wall , genetics , pathogen , gene
Plant cell wall modification is a critical component in stress responses. Endo‐1,4‐β‐glucanases ( EG s) take part in cell wall editing processes, e.g . elongation, ripening and abscission. Here we studied the infection response of S olanum lycopersicum and A rabidopsis thaliana with impaired EG s. Transgenic TomCel1 and TomCel2 tomato antisense plants challenged with P seudomonas syringae showed higher susceptibility, callose priming and increased jasmonic acid pathway marker gene expression. These two EG s could be resistance factors and may act as negative regulators of callose deposition, probably by interfering with the defence‐signalling network. A study of a set of Arabidopsis EG T‐ DNA insertion mutants challenged with P . syringae and B otrytis cinerea revealed that the lack of other EG s interferes with infection phenotype, callose deposition, expression of signalling pathway marker genes and hormonal balance. We conclude that a lack of EG s could alter plant response to pathogens by modifying the properties of the cell wall and/or interfering with signalling pathways, contributing to generate the appropriate signalling outcomes. Analysis of microarray data demonstrates that EG s are differentially expressed upon many different plant–pathogen challenges, hormone treatments and many abiotic stresses. We found some A rabidopsis EG mutants with increased tolerance to osmotic and salt stress. Our results show that impairing EG s can alter plant–pathogen interactions and may contribute to appropriate signalling outcomes in many different biotic and abiotic plant stress responses.