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The expression of the open reading frame of Arabidopsis CAX1, but not its cDNA, confers metal tolerance in yeast
Author(s) -
Shigaki T.,
Mei H.,
Marshall J.,
Li X.,
Manohar M.,
Hirschi K. D.
Publication year - 2010
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/j.1438-8677.2010.00368.x
Subject(s) - biology , open reading frame , yeast , complementary dna , untranslated region , saccharomyces cerevisiae , mutant , phenotype , arabidopsis , genetics , three prime untranslated region , microbiology and biotechnology , messenger rna , gene , peptide sequence
The biochemical properties and regulation of several plant CAX ( CA tion e X changer)‐type vacuolar Ca 2+ /H + exchangers have been extensively analysed in yeast expression assays. In the present study, we compare and contrast the phenotypes of yeast cells expressing the CAX1 cDNA and open reading frame (ORF). We report that the CAX1 ORF, but not the cDNA containing the 3′‐untranslated region (UTR), was able to confer Ca 2+ tolerance when expressed in a Ca 2+ ‐sensitive yeast mutant. Additionally, only yeasts expressing the N‐terminal truncated CAX1 ORF were able to grow on high Mn 2+ media, suggesting that removal of the 3′‐UTR altered activity. However, removal of the 3′‐UTR from another CAX did not alter the yeast phenotypes. Expression studies demonstrated that expressing the CAX1 ORF in yeast elevates CAX1 RNA and protein levels. Our results suggest that the 3′‐UTR modulates expression of CAX1 in yeast.