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Identification of a novel NPR1 ‐like gene from Nicotiana glutinosa and its role in resistance to fungal, bacterial and viral pathogens
Author(s) -
Zhang Y.,
Shi J.,
Liu J.Y.,
Zhang Y.,
Zhang J.D.,
Guo X.Q.
Publication year - 2010
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/j.1438-8677.2009.00210.x
Subject(s) - biology , tobacco mosaic virus , nicotiana tabacum , rhizoctonia solani , systemic acquired resistance , ralstonia solanacearum , nicotiana , gene , plant disease resistance , complementary dna , npr1 , phytophthora , tobacco etch virus , alternaria alternata , microbiology and biotechnology , botany , arabidopsis , genetics , solanaceae , potyvirus , virus , plant virus , pathogen , medicine , heart failure , mutant , natriuretic peptide
The NPR1 or NPR1 ‐like genes play a pivotal role in systemic acquired resistance in plants. Here, we isolated and identified a novel tobacco ( Nicotiana glutinosa ) NPR1 ‐like gene (designated as NgNPR3 ). The full‐length cDNA is 2049 bp in length with a 1767 bp open reading frame which encodes a 588 amino acids protein with an estimated molecular mass of 66 kDa and a calculated pI of 7.14. Homology analysis suggested that the NgNPR3 protein shares significant similarity to AtNPR3 of Arabidopsis . Transient expression assay of NgNPR3‐GFP fusion gene in onion epidermal cells revealed that the NgNPR3 protein was localized to the cytoplasm and moved into the nucleus after redox change. RT‐PCR results indicated that NgNPR3 was up‐regulated after treatment with SA, INA, H 2 O 2, and MeJA, which play important roles in various resistance responses in tobacco. Transcriptional level of NgNPR3 was also up‐regulated after inoculation with Rhizoctonia solani , Phytophthora parasitica , Alternaria alternata , Pseudomonas solanacearum , and potato virus Y (PVY), respectively. When NgNPR3 was overexpressed in N. tabacum cv. Samsun plants, the transgenic plants showed enhanced resistance to the pathogens A. alternate , P. solanacearum and PVY. Furthermore, NgNPR3 ‐mediated disease resistance is dosage‐dependent. Our results suggest that NgNPR3 could be a putative NPR1 ‐like gene, and might play an important role in resistance to a broad range of pathogens in tobacco.