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Identification of potential protein regulators bound to the tissue‐specific positive and negative cis ‐acting elements of a green tissue‐specific promoter in rice
Author(s) -
Cai M.,
Yuan T.,
Duan L.,
Li X.,
Wang S.
Publication year - 2008
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/j.1438-8677.2008.00084.x
Subject(s) - biology , identification (biology) , microbiology and biotechnology , botany
Although some tissue‐specific cis ‐acting elements have been identified, the molecular mechanisms of tissue‐specific gene expression remain elusive. Here, we report the identification by a yeast one‐hybrid screen of five proteins, Os10g31330/glycine‐rich, Os01g10400/metallothionein‐like, Os05g51180/nucleic acid‐binding, Os05g37930/unknown and Os01g01689/phosphatidylinositol kinase that bound to either the positive or negative tissue‐specific cis elements of a rice promoter from the green tissue‐specific D54O gene. These proteins are localised in the nucleus and the genes encoding them are differentially expressed in different tissues, further suggesting their putative roles in regulating gene expression. These results suggest that the green tissue‐specific expression of the D54O gene may be regulated by the interaction of multiple proteins with cis elements in the promoter region.