Premium
Development of Vacuoles and Vacuolar H + ‐ATPase Activity under Extremely High CO 2 Conditions in Chiorococcum littorale Cells
Author(s) -
Sasaki T.,
Pronitalia A.,
Maeshima M.,
Iwasaki I.,
Kurano N.,
Miyachi S.
Publication year - 1999
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/j.1438-8677.1999.tb00710.x
Subject(s) - vacuole , biology , atpase , acclimatization , biochemistry , microbiology and biotechnology , enzyme , botany , cytoplasm
Abstract: The number and cross‐sectional area of vacuoles in Chlorococcum littorale cells visualized with a differential interfer ence fluorescence microscope increased after their transfer from air to 40% CO 2 . An immunological observation indicated that the level of subunit B of vacuolar H*ATPase also increased under 40% CO 2 conditions. The activity of nitrate‐sensitive ATP‐ase associated with the vacuolar membrane was 2–fold higher in 40% CO 2 ‐ ‐ grown cells than in air‐grown cells. The effects of inhi bitors on the ATPase activity confirmed that these activities were derived from vacuolar‐type H‐ATPase. These results sug gest that vacuole development associated with that of vacuolar H + ‐ATPase occurred during the acclimatization of C. littorale cells to extremely high CO 2 conditions.