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Moss ( Physcomitrella patens ) Expressed Sequence Tags Include Several Sequences which are Novel for Plants *
Author(s) -
Reski R.,
Reynolds Susan,
Wehe M.,
KleberJanke Tamara,
Kruse S.
Publication year - 1998
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1998.tb00689.x
Subject(s) - physcomitrella patens , biology , homology (biology) , expressed sequence tag , sequence homology , gene , genetics , complementary dna , homologous recombination , sequence analysis , computational biology , peptide sequence , mutant
The moss Physcomitrella patens (Hedw.) B.S.G. is the first land plant in which gene disruption by homologous recombination Is directly accessible. In order to obtain cloned sequences which may be used in such an approach, complementary DNAs (cDNAs) have been isolated by subtractlve hybridisation of representative cDNA libraries from cytoklnin‐treated tissue. Sequencing of these clones from both ends yielded over 35 kb of non‐redundant sequence Information, of which 20 kb results from clones which appear to be novel to plants. Database comparisons have revealed that 39 of the expressed sequence tags (ESTs) generated show significant homology to identified sequences. Analysis of these ESTs shows a high degree of conservation between Physcomitrella and seed plant sequences, and codon usage is found to be very similar to that In dicotyledonous species. Furthermore, 43 sequences showing no significant homology to sequences in the databases represent previously unidentified expressed genes.