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Gene Control in Somatic Embryos of Digitalis lanata : Expression of the β‐Glucuronidase Gene Fused to a Plastocyanin Promoter
Author(s) -
Thomar S.,
DumkeLehmann Ulrike,
Diettrich Beate,
Luckner M.
Publication year - 1998
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1998.tb00672.x
Subject(s) - biology , microbiology and biotechnology , gus reporter system , reporter gene , gene , embryo , chimeric gene , fusion gene , somatic cell , tapetum , gene expression , genetics , botany , stamen , pollen , microspore
Digitalis lanata was transformed by agrobacteria‐mediated gene transfer with a chimeric reporter gene encoding for β‐glucuronidase (CUS) from Escherichia coll under the control of the plastocyanin 3 (Pc3) promoter from Spinada oleracea (Pc3::uidA fusion gene). Transformed cell lines were regenerated to plants via somatic embryos. CUS activity was determined fluorometrically and histochemically. The Pc3::uidA fusion gene was expressed in the late globular and bipolar stages of somatic embryos. Expression started in globular embryos (stage‐1‐globules) in that part of the parenchymatic tissue which later on formed the cotyledons. No GUS activity was detectable in the parenchymatic tissue forming the root pole, in cells of the developing procambium or in epidermal cells. These tissues were free of GUS activity also in bipolar embryos. The parenchymatic cells of the cotyledons and the primary cortex of the hypocotyl of germinating embryos showed GUS activity, in contrast to the epidermal cells and the cells of the central cylinder.