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The Arabidopsis thaliana AtSUC2 Gene is Specifically Expressed in Companion Cells
Author(s) -
Stadler Ruth,
Sauer N.
Publication year - 1996
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1996.tb00577.x
Subject(s) - arabidopsis , arabidopsis thaliana , sucrose , phloem , green fluorescent protein , gene , fusion protein , biology , polyclonal antibodies , microbiology and biotechnology , biochemistry , genetics , botany , recombinant dna , mutant , antigen
Polyclonal antisera against a fusion protein of β‐galactosidase and the 20 C‐terminal amino acids of the Arabidopsis thaliana sucrose carrier AtSUC2 were used to determine the cellular localization of the AtSUC2 protein. Using fluorescence‐labelling on sections from different organs of Arabidopsis the AtSUC2 protein was immunolocalized exclusively in companion cells. The presented data indicate that phloem loading in Arabidopsis may be catalyzed by the AtSUC2 sucrose carrier which transports sucrose into the companion cells. No evidence for a participation of the second Arabidopsis sucrose transporter AtSUC1 has been obtained.