Premium
Phylogenetic Relationships between some Members of the Subfamily Lamioideae (Family Labiatae) Inferred from Nucleotide Sequences of the rbcL Gene *
Author(s) -
Wink M.,
Kaufmann Martina
Publication year - 1996
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1996.tb00554.x
Subject(s) - biology , subfamily , phylogenetic tree , clade , sensu , maximum parsimony , botany , genetics , gene , genus
Total DNA was extracted from 25 species (8 genera) of the subfamily Lamioideae sensu Erdtman (family Labiatae). Using rbcL‐specific primers, the rbcL gene was amplified by polymerase chain reaction (PCR) and 1420 bp were sequenced directly, or after cloning. RbcL sequences were evaluated with character‐state (maximum parsimony; PAUP) and distance methods (neighbour‐joining; MEGA). In agreement with classical systematics all taxa studied cluster outside the Nepetoideae. The following clades are apparent: I ‐ Westringia/Prostanthera , II ‐ Ajuga/Teucrium and III ‐ Scutellaria, Stachys, Lamium and Marrubium. Lamium album, L. amplexicaule and L. garganicum appear as a sister clade to Marrubium . Both share a common ancestor with a second Lamium clade (including L. purpureum, L. maculatum and L. galeobdolon ). The phylogenetic relationships are discussed with regard to the systematic classification of the subfamilies Lamioideae and Nepetoideae (sensu Erdtman).