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Reversion of deformed ras1 − cells of Schizosaccharomyces pombe to rod‐like cells by nutrient stimulation
Author(s) -
PICHOVÁ ALENA,
VONDRÁKOVÁ DAGMAR,
STREIBLOVÁ EVA
Publication year - 1994
Publication title -
acta botanica neerlandica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0044-5983
DOI - 10.1111/j.1438-8677.1994.tb00734.x
Subject(s) - schizosaccharomyces pombe , reversion , schizosaccharomyces , cell wall , biophysics , cell , microbiology and biotechnology , mutant , yeast , biology , chemistry , biochemistry , phenotype , saccharomyces cerevisiae , gene
SUMMARY The reversion of the highly spherical ras1 − phenotype of the sterile mutant of Schizosaccharomyces pombe was examined by fluorescence microscopy of Calcofluor‐ and rhodamine phalloidin‐labelled cells and by freeze‐fracturing. Rapid and dramatic changes occurred in cell shape after a nutritional stimulus, with cells regaining their rod‐like cell shape during successive cell divisions. This was achieved by restoration of the cell wall architecture and of the invaginated state of the plasma membrane. This is the first demonstration of a nutrient‐induced reversion of ras1 − S. pombe cells from rounded to rod‐like cell shape.