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Paxillus involutus/Pinus sylvestris Mycorrhizae from Heavily Polluted Forest II. Ultrastructural and Cytochemical Observations
Author(s) -
Turnau K.,
Kottke I.,
Dexheimer J.
Publication year - 1994
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1994.tb00411.x
Subject(s) - paxillus involutus , cadmium , botany , biology , ultrastructure , chemistry , mycorrhiza , symbiosis , paleontology , organic chemistry , bacteria
Paxillus involutus/Pinus sylvestris mycorrhizae, collected from experimental plots (situated in a Pino‐Quercetum forest) treated with cadmium dust, were previously reported to accumulate metals like Cd, P, S, N, Al and Si in vacuolar bodies. The Gomori‐Swift test was applied to the same material to reveal if cysteine‐rich proteins might be involved in element detoxification. Vacuolar deposits of high and low phosphate level were both stained. Higher intensity of the Swift reaction was found in the fungal mycelium from mycorrhizae sampled from polluted plots than in mycorrhizae from unpolluted sites. In both cases a higher intensity of the test was revealed in the host cytoplasm close to the Hartig net than in internal parts of roots. Different intensities of the Gomori‐Swift reaction were also observed when the Hartig net was compared to the fungal mantle. A PATAg test was additionally processed and revealed an accumulation of glycogen in rosettes and net‐like structures when samples from cadmium dust treated plots were observed.