Premium
Illustration of the Genetic Differences in Ozone Sensitivity Between the Varieties Nicotiana tabacum Bel W 3 and Bel B Using Various Plant Systems
Author(s) -
Städtler Sabine,
Ziegler H.
Publication year - 1993
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1993.tb00748.x
Subject(s) - fumigation , ozone , nicotiana tabacum , greenhouse , horticulture , biology , botany , chemistry , gene , genetics , organic chemistry
The ozone‐sensitive tobacco variety Bel W 3 was compared with the tolerant cv. Bel B using amphidiploid and amphihaploid genotypes of both. In search of the first genotypical differences, their reaction to acute ozone treatments was investigated with systems of decreasing degree of complexity: whole plants, grown under field, greenhouse and sterile conditions, excised tissues, calli, CCP, MCP and subcellular reactions. It was common to all systems that a fumigation, which clearly exceeds the threshold of the most tolerant material, led to equal reactions in all genotypes in respect of visible injury and membrane leaching. With whole plants and leaf discs growing conditions were found to influence the ozone threshold more than the genotype. Because the most resistant field‐grown plants vary widely in their reaction, only sterile or greenhouse grown genotypes were compared. With the exception of whole sterile plants (no genotypical threshold differences), amphihaploids were more susceptible to ozone than their respective amphidiploids as to threshold and sensitivity spectra in all systems investigated. Higher ozone thresholds were detected for Bel B in all systems with one exception: MCP exhibited a lower threshold but also a lower degree of damage in the first buffer range of the sensitivity spectrum than those of Bel W 3 . Post‐fumigation starch accumulation in mesophyll chloroplasts was the most prominent subcellular ozone reaction.