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Reconstitution into Liposomes of the Tonoplast ATP‐ and Pyrophosphate‐Dependent Proton Pumps from Rubus hispidus Cell Cultures
Author(s) -
Perotti E.,
Chanson A.
Publication year - 1993
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1993.tb00739.x
Subject(s) - pyrophosphate , vesicle , liposome , rubus , proton transport , chemistry , vacuole , biochemistry , diaphragm pump , biophysics , membrane , chromatography , biology , enzyme , botany , cytoplasm , materials science , micropump , nanotechnology
The ATP‐ and pyrophosphate‐dependent proton pumps from tonoplast‐enriched vesicles prepared from Rubus hispidus cell cultures were solubilized in the presence of polyoxyethylene(9,10)p‐t‐octylphenol (Triton X‐100) and reconstituted into liposomes of soybean phospholipids, using Bio‐Beads SM‐2 to remove the detergent. The specific activity of the two pumps was greatly increased by the solubilization‐reconstitution procedure. Identical characteristics were found for pyrophosphate‐dependent proton transport in native and reconstituted vesicles. On the other hand, the ATP‐dependent proton transport of the reconstituted vesicles was no longer inhibited by KNO 3 .