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Sub‐cellular Localization of Calcium in Azolla‐Anabaena Symbiosis by Chlortetracycline, ESI and EELS
Author(s) -
Canini Antonella,
Albertano Patrizia,
Caiola Maria Grilli
Publication year - 1993
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1993.tb00351.x
Subject(s) - cytoplasm , azolla , calcium , heterocyst , anabaena , symbiosis , egta , subcellular localization , biophysics , chemistry , botany , biochemistry , biology , cyanobacteria , bacteria , genetics , organic chemistry
The subcellular localization of calcium in cells of symbiotic partners located within leaf cavities of Azolla was investigated by using chlorotetracycline, ESI and EELS analysis. Loosely membrane‐bound calcium was evidenced by using CTC or EGTA and CTC, in cytoplasmic regions of Azolla hair cells and in cytoplasm of the cyanobiont. Tightly membrane‐bound calcium revealed by CTC, and ESI and EELS analysis, was observed in cyanophycin granules and carboxysomes of the cyanobiont. A third calcium type, revealed by ESI and EELS analysis, was localized at the level of cell walls of simple and branched Azolla hairs, in the envelope of heterocysts, and in the cell walls of the cyanobiont.