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Somatic Hybrids of Sunflower ( Helianthus annuus L.) Identified at the Callus Stage by Isoenzyme Analysis
Author(s) -
Barth S.,
Woeste D.,
Schnabl Heide
Publication year - 1993
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1993.tb00343.x
Subject(s) - helianthus annuus , protoplast , hypocotyl , callus , isozyme , sunflower , hybrid , somatic fusion , biology , cultivar , botany , somatic cell , horticulture , biochemistry , enzyme , gene
Dark grown hypocotyl protoplasts from Helianthus annuus L. cvs. Cerflor and Euroflor were electrically fused to produce somatic hybrids. Following fusion, the protoplasts were cultivated in agarose droplets for four weeks. Macroscopic visible calli (0.1‐0.3 mm) were transferred onto solid medium and calli reaching a size of 3 mm were collected. Their isoenzyme patterns were analysed based on two different isoenzymes that allow discrimination between the two cultivars used for fusion. From the examined calli, about 26 % showed an isoenzyme pattern of putative binary heterokaryocytes. The isoenzyme pattern of the non‐fused control revealed less than 5% of possibly chimeric colonies.