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Ethrel enhances the degradation of artificial membrane vesicles (liposomes) by phospholipase A 2 and phospholipase C
Author(s) -
Reuveni Moshe
Publication year - 1992
Publication title -
acta botanica neerlandica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0044-5983
DOI - 10.1111/j.1438-8677.1992.tb01335.x
Subject(s) - liposome , vesicle , phospholipase , ethylene , chemistry , phospholipase a2 , membrane , phospholipase c , phosphatidylethanolamine , biochemistry , lipid bilayer , phospholipase d , biophysics , enzyme , phosphatidylcholine , phospholipid , biology , catalysis
SUMMARY Ethylene, a lipid‐soluble gas, is known to have considerable effects on plant metabolism. It is possible that some of these metabolic effects are the result of ethylene's lipid solubility. The effect of low concentrations of 2‐chloroethylphosphonic acid (ethrel), an ethylene‐releasing compound, on the susceptibility of artificial membrane vesicles (liposomes) to phospholipase A 2 and phospholipase C was studied. Liposomes were found to be more susceptible to degradation by these two phospholipases in the presence of ethrel. Ethrel promoted maximal degradation of liposomes by phospholipase C at a concentration of 55 n m . Ethrel also enhanced the leakiness of liposomes to l ‐leucine only in the presence of phospholipase A 2 . The rate of degradation of liposomes by both phospholipases was enhanced by about 20–30% in the presence of ethrel. The results may indicate that ethylene may affect the accessibility of phospholipases to their target sites within the lipid bilayer of artificial membrane vesicles.