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In Vitro and in Vivo Phosphorylation of Stomatal Phosphoenolpyruvate Carboxylase from Vicia faba L.
Author(s) -
Schnabl Heide,
Denecke M.,
Schulz M.
Publication year - 1992
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1992.tb00314.x
Subject(s) - vicia faba , phosphoenolpyruvate carboxylase , guard cell , in vivo , biochemistry , ammonium sulfate precipitation , in vitro , biology , phosphorylation , phosphoenolpyruvate carboxykinase , glycine , enzyme , ammonium , pyruvate carboxylase , botany , chemistry , amino acid , microbiology and biotechnology , organic chemistry , size exclusion chromatography
The in vitro and in vivo phosphorylation of stomatal phosphoenolpyruvate carboxylase [EC 4.1. 1.31] from Vicia faba L. was demonstrated by feeding the concentrated enzyme after 0–70% ammonium sulfate precipitation of guard cell protoplasts with 32 P and subsequent analysis of autoradiograms and Western immunoblots, after SDS‐PAGE, of protein samples. The in vitro and in vivo results provide evidence for a radioactive labeling of the two stomatal PEPCase bands (112 and 110 kDa).