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In Vitro Ovary Culture of some Apogon Garden Irises ( Iris pseudacorus L., I. setosa Pall., I. versicolor L.)
Author(s) -
Laublin Geneviève,
Cappadocia M.
Publication year - 1992
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1992.tb00305.x
Subject(s) - biology , explant culture , callus , botany , kinetin , ovary , inflorescence , in vitro , biochemistry , endocrinology
Various organs of immature inflorescences from 32 selected genotypes of Iris versicolor , 6 of I. pseudacorus and 7 of I. setosa were cultured in vitro on two induction media supplemented with 45 μ M 2, 4‐D or 4.5 μ M each of 2, 4‐D and kinetin. Among the explants tested, peduncle sections showed a very limited response, and only ovary sections cultured under low light intensity consistently produced calluses and/or meristematic nodules on both media. This occurred in all genotypes of I. pseudacorus , whereas only a fraction of the genotypes belonging to the other species responded at the induction phase. Responding explants with callus and/or meristematic nodules were cut into halves and transferred onto a regeneration medium containing 22 μ M benzyladenine, which induced shoot formation. Once again, large differences in response were noted among genotypes of each species. Those belonging to I. pseudacorus all gave a positive response producing vigorous shoots. Cytological analyses performed on 62 regenerated plants did not reveal changes in chromosome number.