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Cryopreservation of Embryogenic Suspension Cultures of Barley ( Hordeum vulgare L.) *
Author(s) -
Fretz A.,
Jähne A.,
Lörz H.
Publication year - 1992
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1992.tb00279.x
Subject(s) - plantlet , cryoprotectant , sorbitol , cryopreservation , hordeum vulgare , sucrose , glycerol , botany , suspension culture , biology , stamen , protoplast , suspension (topology) , proline , horticulture , regeneration (biology) , petri dish , chemistry , tissue culture , cell culture , poaceae , embryo , food science , biochemistry , microbiology and biotechnology , pollen , in vitro , genetics , mathematics , amino acid , homotopy , pure mathematics
Anther‐derived suspension cultures of Hordeun vulgare L. were cryopreserved by slow cooling and storage in liquid nitrogen for up to 55 days. Cells were pre‐cultivated in L3 suspension medium supplemented with sorbitol. For freeze preservation the cells were treated with different combinations of cryoprotectant agents such as DMSO, proline, glycerol and sucrose. After rapid thawing high viabilities of up to 77% could be achieved. Cell growth commenced 2‐ 3 weeks later. The frequency of plantlet regeneration was 1%.