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Transformation of a recalcitrant potato cultivar by an Agrobacterium binary vector
Author(s) -
Mlynarova L.,
Bauer M.
Publication year - 1991
Publication title -
acta botanica neerlandica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0044-5983
DOI - 10.1111/j.1438-8677.1991.tb01548.x
Subject(s) - agrobacterium , transformation (genetics) , kanamycin , biology , callus , explant culture , shoot , cultivar , genetically modified crops , plasmid , agrobacterium tumefaciens , southern blot , expression vector , botany , transgene , horticulture , genetics , gene , recombinant dna , in vitro
SUMMARY The CzechoSlovak potato cultivar Lipa was recalcitrant to transformation using established procedures. A modified leaf disc procedure was developed, which allowed the transformation of Lipa with the Agrobacterium Ti plasmid‐based binary vector pARC8. After transformation, leaf explants were incubated on callus‐inducing selection medium containing 50 mg/1 kanamycin for 6 weeks, before selective conditions for shoot induction were applied. Shoot induction was continued for up to 5 months. Transgenic plants were obtained from shoots on selective root‐induction medium. Southern‐blot analysis confirmed the presence of T‐DNA from the vector pARC8 in 1–2 copies per tetraploid genome in all rooted transgenic clones.