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Patch‐clamp studies in cell membranes of higher plants
Author(s) -
Maathuis F. J. M.,
Prins H. B. A.
Publication year - 1991
Publication title -
acta botanica neerlandica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0044-5983
DOI - 10.1111/j.1438-8677.1991.tb01546.x
Subject(s) - ion channel , patch clamp , electrophysiology , conductance , membrane , channel (broadcasting) , biophysics , clamp , membrane potential , ion , chemistry , voltage clamp , biological system , computer science , materials science , neuroscience , biology , physics , biochemistry , telecommunications , receptor , clamping , organic chemistry , computer vision , condensed matter physics
SUMMARY The patch‐clamp technique is a relatively new and powerful electrophysiological technique. Its resolving power is such that ion currents can be recorded through only one protein or ion‐channel. The application of this technique in higher plant physiology, its principles and advantages are discussed in this paper. Furthermore, some actual results of single‐channel recordings, whole cell recordings and ATPase currents are shown and examples are given of ion‐channel conductance and selectivity calculations.