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The effect of colchicine on microtubules and microfibrils in root hairs
Author(s) -
Emons A. M. C.,
WoltersArts A. M. C.,
Traas J. A.,
Derksen J.
Publication year - 1990
Publication title -
acta botanica neerlandica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0044-5983
DOI - 10.1111/j.1438-8677.1990.tb01442.x
Subject(s) - microtubule , colchicine , depolymerization , root hair , raphanus , biophysics , botany , texture (cosmology) , biology , chemistry , microbiology and biotechnology , biochemistry , polymer chemistry , artificial intelligence , gene , computer science , image (mathematics) , genetics
SUMMARY Roots of Equisetum hyemale and Raphanus sativus were grown in concentrations of colchicine that slowed down root hair growth initially, and later stopped growth completely. The treatment effected depolymerization of most of the microtubules. The wall texture in root hairs of E. hyemale was helicoidal after treatment with colchicine, as in untreated hairs. In root hairs of R. sativus the deposition orientation of the microfibrils changed (prior to microtubule depolymerization) from axial to oblique and transverse, but deposition remained ordered. As ordered wall texture is deposited in orientations that differ from the orientation of the depolymerizing microtubules and also in the absence of microtubules, it is concluded that microtubules do not directly control the orientation of nascent microfibrils.