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The Effect of Phosphinothricin (Glufosinate) on Glutathione Synthesis in Plants
Author(s) -
Herold A.,
Wendler C.,
Wild A.
Publication year - 1990
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1990.tb00128.x
Subject(s) - glutamine synthetase , buthionine sulfoximine , glufosinate , glutathione , enzyme , in vivo , methionine , biochemistry , glutathione transferase , glutamine , biology , in vitro , chemistry , amino acid , genetics , glyphosate , agronomy
The inhibitory effect of DL‐phosphinothricin (glufosinate) on glutathione synthesis was studied in vivo and in vitro . The influence of phosphinothricin on γ‐glutamylcysteine synthetase was compared with the already known effects of l ‐buthionine sulfoximine and l ‐methionine sulfoximine. The results showed that phosphinothricin and buthionine sulfoximine are inhibitors of γ‐glutamylcysteine synthetase of plants. With both substances the enzyme was inhibited by 50 % at a concentration of 7 . 10 −4 M (pI 50 = 3.15). Methionine sulfoximine reduced the enzyme activity by 50% at 5 . 10 −2 M (pI 50 = 1.30). It is discussed that the target enzyme of phosphinothricin is the glutamine synthetase whereas the γ‐glutamylcysteine synthetase is only an accessory target.