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Growth of Anacystis in the Presence of Thiosulphate and its Consequences for the Architecture of the Photosynthetic Apparatus
Author(s) -
Koenig F.
Publication year - 1990
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1990.tb00126.x
Subject(s) - thylakoid , photosynthesis , dcmu , photosystem ii , chemistry , context (archaeology) , cyanide , cyanobacteria , photosystem , photosystem i , photosynthetic reaction centre , photochemistry , chlorophyll , pigment , biophysics , chloroplast , biology , biochemistry , botany , bacteria , inorganic chemistry , paleontology , genetics , organic chemistry , gene
With the aim of obtaining information on the degree of flexibility maintained in cyanobacteria in context with their phylogenetic position, Anacystis was grown in the presence of thiosulphate, oxidized in a photosystem I (PSI) dependent reaction (K M 7.4 × 10 −3 M thiosulfate). Besides DBMIB, only o ‐phenanthroline and p ‐hydroxymercuribenzoate blocked thiosulphate‐dependent PSI activity to some extent; iodonitrothymol, DCMU and cyanide had no influence. Growth of Anacystis in the presence of thiosulphate induced a reorganization of the photosynthetic apparatus characterized by a shift in the PSII/PSI ratio in favor of PSI, comparable to low light conditions. Capability for oxygenic photosynthesis never completely disappeared; structural elements of PSII were retained in the membrane to a certain degree. The antenna pigment system signalled high light under conditions of thiosulphate oxidation as judged from the ratio of phycocyanin to chlorophyll. Besides a shift in the ratio of PSII to PSI components, the polypeptide pattern of thylakoids from thiosulphate grown cells shows several additional components compared to the controls and, moreover, higher concentrations of some polypeptides present in the controls, particularly a M r 41000 polypeptide. The process of thiosulphate oxidation appears bound to the thylakoid membrane.