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Turnover of O‐Glucosides of Dihydrozeatin and Dihydrozeatin‐9‐riboside During the Cell Growth Cycle of Photoautotrophic Cell Suspension Cultures of Chenopodium rubrum
Author(s) -
Fußeder A.,
Ziegler P.,
Peters W.,
Beck E.
Publication year - 1989
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1989.tb00114.x
Subject(s) - vacuole , riboside , biochemistry , biology , cell culture , glucoside , brassicaceae , lysis , glycoside , chenopodium , plant cell , chenopodiaceae , chemistry , botany , cytoplasm , weed , medicine , genetics , alternative medicine , pathology , gene
Summary The cellular levels of O‐glucosides of 3 H‐(diH)Z and 3 H‐(diH)[9R]Z, the major short‐term metabolites of 3 H‐(diH)Z having been exogenously supplied to photoautotrophically growing suspension cell cultures of Chenopodium rubrum , decreased significantly during further culture, irrespective of whether the cells were maintained in the stationary phase or were transferred to conditions restoring cell divison. Metabolism of both compounds was more pronounced during the active growth phase than during the stationary phase. The O‐glucosides were converted preferentially to polar compounds of as yet unknown nature, which were partly excreted into the medium. The cellular pools of both glycosides remained compartmented within the vacuole. In contrast to the O‐glycosides, the small cellular pools of the aglycones 3 H‐(diH)Z and 3 H‐(diH)[9R]Z maintained their level during the experimental period of 30 days. Small amounts of the glucosides, as well as of the aglycones, were recovered from the medium and could have resulted from the lysis of a few cells. The results demonstrate, for the first time, that O‐glucosides of cytokinins are not irreversibly deposited within the vacuole of plant cells but may serve to maintain a small, but more or less constant pool of extra‐vacuolar, presumably cytosolic, aglycones. (DiH)Z and its derivatives could be demonstrated to be endogenous cytokinins of Chenopodium rubrum suspension cultured cells occurring along with those of the isopentenyladenine and zeatin types.