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Evidence for the Presence of Chlorophyllide b in the Green Alga Scenedesmus obliquus in vivo
Author(s) -
Kotzabasis Kiriakos,
Senger Horst
Publication year - 1989
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1989.tb00088.x
Subject(s) - pheophorbide a , scenedesmus obliquus , pigment , hydroxylamine , chlorophyll , chemistry , chlorophyll b , chlorophyll a , fluorescence , biology , biochemistry , algae , botany , organic chemistry , physics , quantum mechanics
Chlorophyllide b could be extracted from the wild type of Scenedesmus obliquus and its pigment mutant C‐2A'. Its identity was proved by absorption and fluorescence spectroscopy and by a positive hydroxylamine test. Chlorophyllide b could be transformed into pheophorbide b and methylpheophorbide b. The formation of chlorophyllide b from chlorophyll b by dephytylation with chlorophyllase could be ruled out. The stimulation of chlorophyllide b biosynthesis with o ‐phenanthroline, as described in the literature, could not be confirmed under physiological conditions.