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Total inhibition of wall synthesis by 2‐deoxyglucose and polyoxin D in protoplasts of Schizophyllum commune
Author(s) -
Sietsma J. H.,
Wessels J. G. H.
Publication year - 1988
Publication title -
acta botanica neerlandica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0044-5983
DOI - 10.1111/j.1438-8677.1988.tb01578.x
Subject(s) - schizophyllum commune , protoplast , glucan , chitin , hypha , morphogenesis , deoxyglucose , chemistry , cell wall , biology , biophysics , biochemistry , botany , chitosan , gene
SUMMARY The synthesis of the alkali‐soluble (1→3)‐α‐glucan in the wall of Schizophyllum commune ( s ‐glucan) is completely and specifically inhibited by the addition of 2‐deoxyglucose to regenerating protoplasts. In the presence of this substance the protoplasts were still able to generate hyphae indicating that the (1→3)‐α‐glucan does not play an essential role in hyphal morphogenesis. Specific inhibition of formation of chitin and alkali‐insoluble wall glucan by polyoxin D prevented hyphal morphogenesis and led to osmotically stable cells containing walls essentially consisting of s ‐glucan only. Together, 2‐deoxyglucose and polyoxin D prevented the protoplasts from forming any wall component.