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Microculture and Electrofusion of Defined Protoplasts of the Moss Funaria hygrometrica
Author(s) -
Mejía A.,
Spangenberg G.,
Koop H.U.,
Bopp M.
Publication year - 1988
Publication title -
botanica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0932-8629
DOI - 10.1111/j.1438-8677.1988.tb00028.x
Subject(s) - electrofusion , protoplast , protonema , biology , microculture , botany , moss , cell culture , genetics , materials science , metallurgy
Protoplasts isolated from protonemata of Funaria hygrometrica wild type and developmentally abnormal mutants were individually selected and cultured in microdroplets of defined, unconditioned culture medium. Up to 60 % of the microcultured wild type protoplasts regenerated whole plants after 4 weeks. Electrofusion in different combinations of defined protoplast pairs of wild type and mutated forms was performed. The products derived from one‐to‐one electric field induced fusion were efficiently microcultured and somatic hybrids were regenerated.