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IN VITRO GERMINATION AND POLLEN TUBE GROWTH OF MAIZE (ZEA MAYS L.) POLLEN. X. POLLEN SOURCE GENOTYPE AND GIBBERELLIN A 3 INTERACTIONS *
Author(s) -
Pfahler P. L.,
Wilcox M.,
Mulcahy D. L.,
Knauft D. A.
Publication year - 1982
Publication title -
acta botanica neerlandica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0044-5983
DOI - 10.1111/j.1438-8677.1982.tb01596.x
Subject(s) - pollen , germination , pollen tube , gibberellin , biology , agar , sucrose , inoculation , botany , pollen source , horticulture , genotype , pollination , food science , biochemistry , genetics , pollinator , bacteria , gene
SUMMARY Pollen grains from four pollen source genotypes (H55, Oh43, K64 × K55, Ky49 × Ky27) were cultured on an artificial medium (15% sucrose, 0.6% bacto‐agar, 0.03% calcium nitrate, 0.01% boric acid) supplemented with four concentrations (0, 5, 25, 100 ppm) of gibberellin A 3 (GA 3 ). At 1, 2, and 3 h after inoculation, germination percent and pollen tube length were measured. A highly significant concentration x pollen source genotype interaction for germination percent was obtained. Depending on the pollen source genotype, increasing GA 3 concentrations either substantially increased or did not alter germination percent. For pollen tube length, a highly significant concentration x pollen source genotype interaction was also obtained. However, depending on the pollen source genotype, increasing GA 3 concentrations either increased, decreased, or had no effect on pollen tube length. Any stimulatory effect of GA 3 on pollen tube length was expressed early in the germination process or between inoculation and one h after inoculation. The results suggested that heterozygosity level could be a contributing factor in the GA 3 response and within each pollen source genotype, the effects of GA 3 on germination percent and pollen tube length were independent.