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THE HISTOGENESIS OF CALLUS IN COFFEA CANEPHORA STEM EXPLANTS AND THE DISCOVERY OF EARLY EMBRYOID INITIATION
Author(s) -
Nassuth A.,
Wormer T. M.,
Bouman F.,
Staritsky G.
Publication year - 1980
Publication title -
acta botanica neerlandica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0044-5983
DOI - 10.1111/j.1438-8677.1980.tb01187.x
Subject(s) - callus , explant culture , biology , cambium , coffea canephora , botany , stele , histogenesis , epidermis (zoology) , anatomy , xylem , in vitro , coffea arabica , biochemistry , immunohistochemistry , immunology
SUMMARY In stem explants of Coffea canephora all parenchymatic tissues between epidermis and vascular cambium were found to be capable of being transformed into callus tissue, the cortex being the most active layer in this respect. The initial callus cells enlarge and divide periclinally. As a result, the epidermis is ruptured so that the callus emerges. The possible rols of the parenchymatic tissues of the stele in the formation of callus occasionally observed on the cut surfaces of the explants has not been further investigated. After 14 days of incubation proembryo‐like structures could already be observed in the zone of callus formation.