Premium
ON THE RELATIVE EXTRACT ABILITY OF CHLOROPHYL a 685 AND CHLOROPHYLL b COMPLEXES BY TWO DETERGENTS
Author(s) -
Thomas J. B.,
Hammans J. W. Kleinen,
Verwer W.
Publication year - 1977
Publication title -
acta botanica neerlandica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0044-5983
DOI - 10.1111/j.1438-8677.1977.tb01122.x
Subject(s) - digitonin , chlorophyll , chlorophyll a , phaseolus , chemistry , chlorophyll b , chloroplast , sodium dodecyl sulfate , pigment , solubilization , biochemistry , chromatography , botany , biology , enzyme , organic chemistry , gene
SUMMARY Treatment of Phaseolus vulgaris chloroplast fragments with 8 or 4 mM digitonin as well as 8 or 4 mM sodium dodecyl sulfate results in a proportionally higher solubilization of the chlorophyll a 685 complex and a lower one of chlorophyll b as compared to the extractability of the remaining chlorophyll a forms. The results are discussed with respect to previous data suggesting the occurrence of a chlorophyll b ‐chlorophyll a 685‐protein complex. It may be that both pigment forms are bound to different polypeptides of this complex.